تاثیر تاریخ های مختلف کاشت و کاربرد جنیستئین بر مقدار نیتروژن و گره زایی سه گونه یونجه یک ساله

In order to study the effect of planting dates and genistein on nitrogen content and nodulation of three annual Medicago species (Medicago polymorpha cv. Santiago M. rigidula cv. Ragidula, and M. radiata cv. Radiata), an experimental study was conducted during 2004-2005 on the Research Farm of Faculty of Agriculture, Tarbiat Modares University, Tehran (35°43′N and 51°8′E). The factors were arranged as split-split plot in a randomized complete block design with four replications. Planting dates (February 20, March 1 and 11) were randomized to main plots and three annual medics were located in sub-plots, and genistein (0 and 20 μM) was randomized to sub-sub-plot units. Plant nitrogen contnt, nodulation and other traits were significantly different in species and M. polymorpha was better than other species in view of dry nodule weight, nodule number, nodule number in each cluster, nodule cluster number and nodule diameter. Medicago rigidula had more resistance to cold than other varieties, and its forage yield and nitrogen percentage were better than M. polymorpha. Therefore, M. rigidula may be better suited for cold zones. Twenty μmol genistein had remarkable effect on nodulation and nitrogen percentage of annual medics in comparison with control. The result showed that genistein modified negative effect of low temperature environment on nodulation and nitrogen percentage of annual medics. Nodulation and nitrogen percentage increased in all varieties at the first planting date. This finding emphasizes that genistein has a considerable effect on cold resistance establishment in varieties for improving nodulation and increasing plant nitrogen percentage in farm condition.